Wilhelm Weber entered the University of Halle in 1822 where he was taught and strongly influenced by the physicist Johann S C Schweigger and the mathematician Johann Friedrich Pfaff. He wrote his doctoral dissertation under Schweigger's supervision on the theory of reed organ pipes and submitted it to Halle in 1826. After that he taught at Halle from 1827 after completing his habilitation thesis on reed organ pipes as coupled oscillators with acoustic coupling of tongue and air cavity. He published a series of papers on this topic between 1828 and 1830 in Annalen der Physik und Chemie :-
One of the subjects treated was the use of this coupling to maintain constancy of pitch of a pipe under different intensities of blowing, and the possibility that this might provide an improved standard of pitch.
His promotion was rapid for after his appointment as a Privatdozent in 1827 he became an Extraordinary Professor of natural philosophy at Halle in the following year. In fact 1828 was quite a significant year for Weber for in September of that year he and his brother Ernst travelled to Berlin to attend the 7th meeting of the Gesellschaft Deutscher Naturforscher und Arzte. The meeting was organised by Alexander von Humboldt who was very impressed with the talk Weber gave on organ pipes. Equally important was the fact that Carl Friedrich Gauss also attended Weber's lecture and immediately saw the tremendous potential displayed by the young physicist. At this time Gauss was interested in geomagnetism and he realised that Weber would make an outstanding co-worker. He spoke to Weber and asked if he would be interested in taking a position in Göttingen if one were to become available. Indeed after the death of Tobias Mayer Jr, Weber was offered a professorship in physics at Göttingen in April 1831 which he immediately accepted. There followed six years of close friendship and collaboration between Weber and Gauss. He soon gained an excellent reputation as a lecturer, illustrating his lectures with experiments. He felt, however, that students could only learn by doing experiments, not simply by watching them carried out, and he opened the physical laboratory at Göttingen for student use.

In 1832 Weber and Gauss published a joint paper which introduced absolute units of measurement of magnetism for the first time. Before this major advance, measurements were made with a pre-calibrated magnetic instrument and were not properly reproducible. This represented an important step forward in the development of magnetism. Weber made major contributions to this work, particularly by developing sensitive magnetometers and other magnetic instruments. Equally important was Weber's later work extending these ideas on magnetic measurements to electrical measurements which we mention again below. This work led to Maxwell's introduction of some aspects of Weber's distant-action theory into his field theory of electricity and magnetism, see. Another joint venture by Weber and Gauss of fundamental importance was their founding of the Göttingen Magnetische Verein in 1833. In the Gauss-Weber telegraph design is discussed. This telegraph was a battery operated line 3000 metres long connecting the Physical Laboratory and the Astronomical Observatory at Göttingen, allowing simultaneous magnetic observations at the two sites. Gauss and Weber jointly published Atlas Des Erdmagnetismus: Nach Den Elementen Der Theorie Entworfen in 1840 which contains magnetic maps constructed using a network of magnetic observatories which they had organized from 1836 onwards to correlate measurements of terrestrial magnetism around the world.

Not all Weber's work during this time was with Gauss, for he also collaborated with his younger brother Eduard, an anatomist and physiologist, who was interested in the physics of human locomotion, particularly the mechanism of walking. They published the joint work Mechanik der menschlichen Gehwerkzenge in 1836. Weber also published several important papers on acoustics during these years.

Political events had already had a major impact on Weber's life when as a boy his family had to leave Wittenberg. After six highly productive years at Göttingen, events again conspired to alter the direction of his life. To understand these events we need to look briefly at the history of Hanover which had come under British influence after the fall of Napoleon in 1814. George IV imposed a constitution on Hanover in 1819 which meant it was dominated by its nobles. An uprising in 1830, shortly before Weber moved to Göttingen, led to William IV introducing a much more liberal and acceptable constitution in 1833. However, William IV died in June 1837 and Hanover separated from Britain with Victoria becoming Queen of Britain while her uncle Ernest Augustus became King of Hanover. As King he repealed the constitution of 1833, which he considered far too liberal. Two weeks after this act by the King, seven professors from Göttingen sent a protest letter to the King explaining that the oath they had taken as professors bound them to the 1833 constitution. Weber was one of the "Göttingen Seven" who signed the protest and among the others were the brothers Jacob and Wilhelm Grimm, the authors of 'Grimm's Fairy Tales'. As explained in Encyclopaedia Britannica:-
Through their part in this protest directed against despotic authority, [the Göttingen Seven] clearly demonstrated the academic's sense of civil responsibilities, manifesting their own liberal convictions at the same time.
All seven professors were dismissed while three of them were ordered to leave the kingdom of Hanover. Weber, although dismissed, was not forced to leave Göttingen and he continued to work at the Göttingen Magnetische Verein without holdig any university position. Gauss and von Humboldt appealed to the King to reinstate Weber and the King agreed to do so provided Weber make a public retraction of the views expressed in the letter of protest. Weber, however, was a man of strong principles and he was certainly not prepared to make a public statement which went totally against his views so he refused to make the required public retraction. He remained at Göttingen without a position until 1843 but he did take the opportunity to travel between March and August 1838. He first visited Berlin before travelling on to London, where he enjoyed useful conversations many English scientists including John Herschel, and finally to Paris where he met most of the leading French scientists. In 1843 he became professor of physics at Leipzig joining his brothers Ernst and Eduard who were both professors at the University. He was appointed to fill G T Fechner's chair at Leipzig after Fechner had to take retirement due to blindness. At Leipzig, Weber continued the work on Ampère's law of electrical force which he had been undertaking in Göttingen from 1832 onwards. He published Elektrodynamische Massenbestimmungen in 1846:-
Weber's greatest theoretical contributions appeared in the 'Elektrodynamische Massenbestimmungen' Ⓣ, seven long works published from 1846 to 1878, besides a manuscript published posthumously. In the first of these, Weber introduced his dynamometer to test Ampère's law of force between electric current elements, to a degree of precision exceeding Ampère's, and also investigated electromagnetic induction.
Dolbear writes:-
Until Weber's work there had been no such thing as electrical measurements. There had been nothing more than comparisons between magnitudes of the same kind. Weber showed how an electrical quantity could be stated in terms of the unit of time, length, and mass, without any reference to other electrical phenomena, and this was a new and great achievement. The British Association Committee on Electrical Standards adopted Weber's work as a basis for their standards of units. Secondly, he was one of the first to feel the necessity for an adequate mechanical conception of electro-magnetic phenomena, and he worked out in a mathematical way, and gave consistency to the idea of molecular magnets, that is, that every molecule of iron is a magnet by constitution, and the various phenomena of the magnetic field are due to the relative positions of these molecules.
D'Agostino writes:-
Gauss and Weber's systematic assignment of absolute units, overshadowed by our four-unit systems, allowed the nineteenth-century analytic formulation of physical laws (a fundamental requisite for theoretical predictions) ...
In fact what Weber achieved was a bringing together of three laws, namely that describing the interactions of two electric charges at rest, Ampère's law for moving electric currents, and the law describing electrical induction.

In 1848 a series of republican revolts against European monarchies spread through France, Germany, Italy, and the Austrian Empire. Ernest Augustus's reign was already a stormy one with trouble between the King and his people and the 1848 revolt forced him to grant Hanover a new much more liberal constitution. The door was thus opened for Weber to return to Göttingen but his position had already been filled by Johann Benedict Listing in 1839 despite the fact that he had never published a paper. Weber insisted that Listing should keep the chair so he returned to Göttingen in 1849 as the Director of the Astronomical Observatory. By this time Gauss was over seventy years of age and rather too old for the two scientists to restart the remarkably fruitful collaboration which had begun nearly twenty years earlier. Gauss died in 1855, and shortly before this Weber began a collaboration with Rudolph Hermann Arndt Kohlrausch who was then at Marburg. Their work on the ratio between the electrodynamic and electrostatic units of charge, published in 1856, proved extremely important and was crucial to Maxwell in his electromagnetic theory of light. Weber found the ratio was  3.1074×10⁸m/sec but failed to take any notice of the fact that this was close to the speed of light. In fact the first use of "c" for the speed of light appears in this paper. Bernhard Riemann, who spent eighteen months as Weber's assistant, was present when the experiments were carried out and he did make the connection between light and both electrodynamic and electromagnetic phenomena.

Weber's later years at Göttingen were devoted to work in electrodynamics and the electrical structure of matter. He was described by Thomas Hirst, who visited Göttingen in the 1850s, in the following way:-
He speaks and stutters on unceasingly, one has nothing to do but listen. Sometimes he laughs for no earthly reason, and one feels sorry at being not able to join him.
Woodruff describes Weber as:-
... friendly, modest and unsophisticated.
Weber never married but his sister often helped manage his household and, in later years, his niece carried out this task. His pleasures outside of his academic work included hiking and he loved to walk for long distances. He died peacefully in the garden of his home in Göttingen having outlived both his scientist brothers, the elder by 13 years and the younger by 20 years. He is buried in the same cemetery as two famous physicists Max Planck and Max Born.

For his outstanding achievements Weber received many honours. He was elected to the Royal Society of London in 1850 and awarded their Copley Medal in 1859. He was elected an honorary fellow of the Royal Society of Edinburgh on 2 March 1874. He was also elected to the American Academy of Arts and Sciences. In 1879 he was awarded the Matteucci Medal by the Italian Society of Sciences. In 1935 the unit of magnetic flux was named the weber in his honour. Actually this is not quite as straightforward as it might at first appear. The unit of electric current is known as the ampere, the term becoming accepted after being proposed by Helmholtz in 1881. In fact around that time the term weber was quite widely used for the unit of electric current but Helmholtz had been in a number of disputes with Weber so he was keen to not have such an important unit named after someone with whom he frequently disagreed. In particular Helmholtz and Weber held different ideas about the nature of the mass of electric corpuscles; see for example [9]. In fact A E Woodruff, in a review of, writes:-
It is the concept of electrical atomicity, as developed by Weber and elaborated by his followers, which represents his chief conceptual contribution to the growth of physics.
Writing in, Woodruff expresses these views in the following way:-
Although he was perhaps most widely known during his life for his law of force, which was discarded with the triumph of Maxwell's field theory. Weber left his more lasting impression on physical theory with his atomistic conception of electrical charge and his vision of the role of such charges in determining the electrical, magnetic and thermal properties of matter.